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Image Search Results
Journal: Journal of cellular and molecular medicine
Article Title: DDR1 Targeting HOXA6 Facilitates Bladder Cancer Progression via Inhibiting Ferroptosis.
doi: 10.1111/jcmm.70410
Figure Lengend Snippet: FIGURE 3 | DDR1 inhibits ferroptosis of BC cells. (A) CCK-8 assay of TCCSUP cells treated with erastin, apoptosis inhibitor ZVAD-FMK, necro- ptosis inhibitor necrosulfonamide or ferroptosis inhibitor ferrostatin-1 combined with NC and OE-DDR1. (B) CCK-8 assay of T24 cells treated with erastin, apoptosis inhibitor ZVAD-FMK, necroptosis inhibitor necrosulfonamide or ferroptosis inhibitor ferrostatin-1 combined with NC and OE- DDR1. (C) Levels of glutathione (GSH), malondialdehyde (MDA) and Fe2+ in TCCSUP cells. (D) Levels of GSH, MDA and Fe2+ in T24 cells. (E) RT- qPCR analysis of glutathione peroxidase 4 (GPX4), solute carrier family 7 member 11 (SLC7A11) and acyl-CoA synthetase long chain family member 4 (ACSL4) expression in TCCSUP cells. (F) RT-qPCR analysis of GPX4, SLC7A11 and ACSL4 expression in T24 cells. (G) WB analysis and quantifi- cation of SLC7A11, zinc finger E-box binding homeobox 1 (ZEB1), E-cadherin, ACSL4, GPX4, yes-associated protein (YAP), p-YAP, neurofibromin 2 (NF2), and p-NF2 expression in TCCSUP and T24 cells. N = 3. *p < 0.05 versus control group. #p < 0.05 versus NC + erastin or si-NC + erastin group.
Article Snippet: Then, the sections were blocked with goat serum at room temperature for 10 min, and then incubated with the primary antibodies against GPX4 (#Ab125066, Abcam), SLC7A11 (#Ab175186, Abcam), ACSL4 (#Ab155282, Abcam), ZEB1 (#Sc- 515,797, Santa Cruz Biotechnology), E- cadherin (#20874- 1- AP, Proteintech), p- YAP (#29018- 1- AP, Proteintech) and
Techniques: CCK-8 Assay, Quantitative RT-PCR, Expressing, Binding Assay, Control
Journal: Journal of cellular and molecular medicine
Article Title: DDR1 Targeting HOXA6 Facilitates Bladder Cancer Progression via Inhibiting Ferroptosis.
doi: 10.1111/jcmm.70410
Figure Lengend Snippet: FIGURE 8 | Effect of DDR1 on the levels of proteins involved in ferroptosis, EMT and NF2–YAP pathway in T24 xenografts. Representative IHC staining and quantification of GPX4, SLC7A11, ACSL4, ZEB1, E-cadherin, p-YAP and p-NF2 in T24 xenograft group tumours. N = 3. *p < 0.05 versus control group. *p < 0.05 versus control group. #p < 0.05 versus erastin group. $p < 0.05 versus OE-DDR1 + erastin group.
Article Snippet: Then, the sections were blocked with goat serum at room temperature for 10 min, and then incubated with the primary antibodies against GPX4 (#Ab125066, Abcam), SLC7A11 (#Ab175186, Abcam), ACSL4 (#Ab155282, Abcam), ZEB1 (#Sc- 515,797, Santa Cruz Biotechnology), E- cadherin (#20874- 1- AP, Proteintech), p- YAP (#29018- 1- AP, Proteintech) and
Techniques: Immunohistochemistry, Control
Journal: Environmental health and preventive medicine
Article Title: Lead exposure promotes NF2-wildtype meningioma cell proliferation through the Merlin-Hippo signaling pathway.
doi: 10.1265/ehpm.24-00216
Figure Lengend Snippet: Fig. 4 Merlin expression in meningioma cells after lead exposure. (A). Detection of Merlin protein in IOMM-Lee and CH157-MN cells. (B) Detection of Merlin and phosphorylated Merlin (Ser518) after lead treatment in IOMM-Lee cells. (C) Quantification of relative expression levels of Merlin normalized to GAPDH. (D) Quantification of relative expression levels of p-Merlin (Ser518) normalized to total Merlin. Data represents the mean + SEM. Statistical difference was determined using one-way ANOVA test with n = 3–8 samples per group (* p < 0.05; ** p < 0.01).
Article Snippet: Nuclear protein and cytoplasmic protein extraction kit (P0028) was from China Beyotime Inc. Merlin antibody (#9168),
Techniques: Expressing